› 2rgv

X-ray diffraction
2.05Å resolution

The crystal structure of PerR-Ox highlights 2-oxo-Histidine formation

Released:
DOI: 10.2210/pdb2rgv/pdb
PDB entry 2rgv coloured by chain, front view.
PDB entry 2rgv coloured by chain, side view.
PDB entry 2rgv coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structural and functional characterization of 2-oxo-histidine in oxidized PerR protein.
Traoré DA, El Ghazouani A, Jacquamet L, Borel F, Ferrer JL, Lascoux D, Ravanat JL, Jaquinod M, Blondin G, Caux-Thang C, Duarte V, Latour JM
Nat Chem Biol 5 53-9 (2009)
PMID: 19079268

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159778 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxide operon regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 145 amino acids
Theoretical weight: 16.49 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P71086 (Residues: 1-145; Coverage: 100%)
Gene names: BSU08730, perR, ygaG
Sequence domains: Ferric uptake regulator family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand OHI 6 x OHI

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P1
Unit cell:
a: 41.25Å b: 42.7Å c: 53.02Å
α: 82.05° β: 79.98° γ: 61.63°
R-values:
R R work R free
0.216 0.211 0.308
Expression system: Escherichia coli

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Citations

23 review citations

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
22 more

4 mentions without citation

Peroxide-sensing transcriptional regulators in bacteria.
Dubbs et al. (2012)
3 more