› 2rgy

X-ray diffraction
2.05Å resolution

Crystal structure of transcriptional regulator of LacI family from Burkhoderia phymatum

Released:
DOI: 10.2210/pdb2rgy/pdb
PDB entry 2rgy coloured by chain, front view.
PDB entry 2rgy coloured by chain, side view.
PDB entry 2rgy coloured by chain, top view.
Source organism: Paraburkholderia phymatum STM815
Entry authors: Agarwal R, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
L-alanine = D-alanine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109663 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH lacI-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 32.17 KDa
Source organism: Paraburkholderia phymatum STM815
Expression system: Escherichia coli
UniProt:
  • Canonical: B2JNW3 (Residues: 55-333; Coverage: 84%)
Gene name: Bphy_5439
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2221
Unit cell:
a: 58.543Å b: 118.777Å c: 85.439Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.267
Expression system: Escherichia coli

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