› 2rh7

X-ray diffraction
1.5Å resolution

Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla Reniformis

Released:
DOI: 10.2210/pdb2rh7/pdb
PDB entry 2rh7 coloured by chain, front view.
PDB entry 2rh7 coloured by chain, side view.
PDB entry 2rh7 coloured by chain, top view.
Source organism: Renilla reniformis
Primary publication:
Crystal structures of the luciferase and green fluorescent protein from Renilla reniformis.
Loening AM, Fenn TD, Gambhir SS
J Mol Biol 374 1017-28 (2007)
PMID: 17980388

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188306 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 239 amino acids
Theoretical weight: 27.06 KDa
Source organism: Renilla reniformis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q963I9 (Residues: 1-233; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 4 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21212
Unit cell:
a: 73.744Å b: 85.405Å c: 158.425Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.291 0.291 0.325
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
16 more

4 mentions without citation

Quaternary structure of a G-protein-coupled receptor heterotetramer in complex with Gi and Gs.
Navarro et al. (2016)
3 more