› 2rh8

X-ray diffraction
2.22Å resolution

Structure of apo anthocyanidin reductase from vitis vinifera

Released:
DOI: 10.2210/pdb2rh8/pdb
PDB entry 2rh8 coloured by chain, front view.
PDB entry 2rh8 coloured by chain, side view.
PDB entry 2rh8 coloured by chain, top view.
Source organism: Vitis vinifera
Primary publication:
Structure and epimerase activity of anthocyanidin reductase from Vitis vinifera.
Gargouri M, Manigand C, Maugé C, Granier T, Langlois d'Estaintot B, Cala O, Pianet I, Bathany K, Chaudière J, Gallois B
Acta Crystallogr D Biol Crystallogr 65 989-1000 (2009)
PMID: 19690377

Function and Biology Details

Reaction catalysed: 
A (2S,3R)-flavan-3-ol + 2 NADP(+) = an anthocyanidin with a 3-hydroxy group + 2 NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177093 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anthocyanidin reductase ((2S)-flavan-3-ol-forming) Chain: A
Molecule details ›
Chain: A
Length: 338 amino acids
Theoretical weight: 36.8 KDa
Source organism: Vitis vinifera
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5FB34 (Residues: 1-338; Coverage: 100%)
Gene name: ANR
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P6122
Unit cell:
a: 50.47Å b: 50.47Å c: 440.406Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.199 0.27
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Biosynthesis of anthocyanins and their regulation in colored grapes.
He et al. (2010)
4 more

5 mentions without citation

Structure-based computational study of two disease resistance gene homologues (Hm1 and Hm2) in maize (Zea mays L.) with implications in plant-pathogen interactions.
Dehury et al. (2014)
4 more