› 2rjz

X-ray diffraction
2.2Å resolution

Crystal structure of the type 4 fimbrial biogenesis protein PilO from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb2rjz/pdb
PDB entry 2rjz coloured by chain, front view.
PDB entry 2rjz coloured by chain, side view.
PDB entry 2rjz coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Periplasmic domains of Pseudomonas aeruginosa PilN and PilO form a stable heterodimeric complex.
Sampaleanu LM, Bonanno JB, Ayers M, Koo J, Tammam S, Burley SK, Almo SC, Burrows LL, Howell PL
J Mol Biol 394 143-59 (2009)
PMID: 19857646

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175918 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PilO protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 147 amino acids
Theoretical weight: 16.44 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q51353 (Residues: 69-204; Coverage: 66%)
Gene names: ALP65_02009, CAZ10_01245, DT376_01075, GNQ48_04070, GUL26_08175, IPC1295_08185, NCTC13621_05618, PAERUG_P19_London_7_VIM_2_05_10_03102, pilO
Sequence domains: Pilus assembly protein, PilO
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P64
Unit cell:
a: 70.471Å b: 70.471Å c: 116.876Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.193 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Pseudomonas aeruginosa twitching motility: type IV pili in action.
Burrows LL. (2012)
9 more

6 mentions without citation

Structural insights into the Type II secretion nanomachine.
McLaughlin et al. (2012)
5 more