› 2rko

X-ray diffraction
3.35Å resolution

Crystal Structure of the Vps4p-dimer

Released:
DOI: 10.2210/pdb2rko/pdb
PDB entry 2rko coloured by chain, front view.
PDB entry 2rko coloured by chain, side view.
PDB entry 2rko coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Vacuolar protein sorting: two different functional states of the AAA-ATPase Vps4p.
Hartmann C, Chami M, Zachariae U, de Groot BL, Engel A, Grütter MG
J Mol Biol 377 352-63 (2008)
PMID: 18272179

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156662 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vacuolar protein sorting-associated protein 4 Chain: A
Molecule details ›
Chain: A
Length: 331 amino acids
Theoretical weight: 36.65 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P52917 (Residues: 124-437; Coverage: 72%)
Gene names: CSC1, DID6, END13, GRD13, P9705.10, VPL4, VPS4, VPT10, YPR173C
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P6522
Unit cell:
a: 109.116Å b: 109.116Å c: 176.632Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.27 0.268 0.298
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Membrane budding and scission by the ESCRT machinery: it's all in the neck.
Hurley et al. (2010)
9 more

2 mentions without citation

Biochemical and structural studies of yeast Vps4 oligomerization.
Gonciarz et al. (2008)
1 more