› 2rl7

X-ray diffraction
2Å resolution

Crystal Structure cation-dependent mannose 6-phosphate receptor at pH 4.8

Released:
DOI: 10.2210/pdb2rl7/pdb
PDB entry 2rl7 coloured by chain, front view.
PDB entry 2rl7 coloured by chain, side view.
PDB entry 2rl7 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural insights into the mechanism of pH-dependent ligand binding and release by the cation-dependent mannose 6-phosphate receptor.
Olson LJ, Hindsgaul O, Dahms NM, Kim JJ
J Biol Chem 283 10124-34 (2008)
PMID: 18272523

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145846 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Cation-dependent mannose-6-phosphate receptor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 154 amino acids
Theoretical weight: 17.44 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P11456 (Residues: 29-182; Coverage: 61%)
Gene name: M6PR
Sequence domains: Mannose-6-phosphate receptor
Structure domains: Mannose-6-phosphate receptor binding domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand GOL 2 x GOL
Ligand ACT 5 x ACT
Ligand CAC 1 x CAC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 50.84Å b: 123.028Å c: 53.092Å
α: 90° β: 92.46° γ: 90°
R-values:
R R work R free
0.211 0.211 0.257
Expression system: Trichoplusia ni

Quick links

Citations

7 review citations

The repertoire of glycan determinants in the human glycome.
Cummings RD. (2009)
6 more

3 mentions without citation

Strategies for carbohydrate recognition by the mannose 6-phosphate receptors.
Dahms et al. (2008)
2 more