› 2rl8

X-ray diffraction
1.45Å resolution

Crystal Structure cation-dependent mannose 6-phosphate receptor at pH 6.5 bound to M6P

Released:
DOI: 10.2210/pdb2rl8/pdb
PDB entry 2rl8 coloured by chain, front view.
PDB entry 2rl8 coloured by chain, side view.
PDB entry 2rl8 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural insights into the mechanism of pH-dependent ligand binding and release by the cation-dependent mannose 6-phosphate receptor.
Olson LJ, Hindsgaul O, Dahms NM, Kim JJ
J Biol Chem 283 10124-34 (2008)
PMID: 18272523

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145846 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cation-dependent mannose-6-phosphate receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 154 amino acids
Theoretical weight: 17.44 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P11456 (Residues: 29-182; Coverage: 61%)
Gene name: M6PR
Sequence domains: Mannose-6-phosphate receptor
Structure domains: Mannose-6-phosphate receptor binding domain

Ligands and Environments

3 bound ligands:
Ligand NAG 2 x NAG
Ligand MN 2 x MN
Ligand M6D 2 x M6D
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 91.659Å b: 91.659Å c: 85.079Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.237
Expression system: Trichoplusia ni

Quick links

Citations

7 review citations

The repertoire of glycan determinants in the human glycome.
Cummings RD. (2009)
6 more

3 mentions without citation

Strategies for carbohydrate recognition by the mannose 6-phosphate receptors.
Dahms et al. (2008)
2 more