› 2rln

X-ray diffraction
1.85Å resolution

THERMODYNAMIC AND STRUCTURAL CONSEQUENCES OF CHANGING A SULPHUR ATOM TO A METHYLENE GROUP IN THE M13NLE MUTATION IN RIBONUCLEASE S

Released:
DOI: 10.2210/pdb2rln/pdb
PDB entry 2rln coloured by chain, front view.
PDB entry 2rln coloured by chain, side view.
PDB entry 2rln coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Thermodynamic and structural consequences of changing a sulfur atom to a methylene group in the M13Nle mutation in ribonuclease-S.
Thomson J, Ratnaparkhi GS, Varadarajan R, Sturtevant JM, Richards FM
Biochemistry 33 8587-93 (1994)
PMID: 8031793

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158391 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribonuclease pancreatic Chain: S
Molecule details ›
Chain: S
Length: 16 amino acids
Theoretical weight: 1.73 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P61823 (Residues: 27-41; Coverage: 12%)
Gene names: RNASE1, RNS1
Ribonuclease pancreatic Chain: E
Molecule details ›
Chain: E
Length: 109 amino acids
Theoretical weight: 11.97 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P61823 (Residues: 42-150; Coverage: 88%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand NLE 1 x NLE

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 44.74Å b: 44.74Å c: 97.35Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.174 not available
Expression system: Not provided

Quick links

Citations

8 review citations

Sensing the heat: the application of isothermal titration calorimetry to thermodynamic studies of biomolecular interactions.
Ladbury et al. (1996)
7 more

1 mention without citation

Peptide Folding and Binding Probed by Systematic Non-canonical Mutagenesis.
Rogers JM. (2020)