› 2ron

Solution NMR

The external thioesterase of the Surfactin-Synthetase

Released:
DOI: 10.2210/pdb2ron/pdb
PDB entry 2ron coloured by chain, ensemble of 20 models, front view.
PDB entry 2ron coloured by chain, ensemble of 20 models, side view.
PDB entry 2ron coloured by chain, ensemble of 20 models, top view.
Source organism: Bacillus subtilis
Primary publication:
Structural basis for the selectivity of the external thioesterase of the surfactin synthetase.
Koglin A, Löhr F, Bernhard F, Rogov VV, Frueh DP, Strieter ER, Mofid MR, Güntert P, Wagner G, Walsh CT, Marahiel MA, Dötsch V
Nature 454 907-11 (2008)
PMID: 18704089

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170693 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Surfactin synthase thioesterase subunit Chain: A
Molecule details ›
Chain: A
Length: 242 amino acids
Theoretical weight: 27.65 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08788 (Residues: 1-242; Coverage: 100%)
Gene names: BSU03520, srfA4, srfAD
Sequence domains: Thioesterase domain
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

24 review citations

The role of dynamic conformational ensembles in biomolecular recognition.
Boehr et al. (2009)
23 more

6 mentions without citation

Structural insights into nonribosomal peptide enzymatic assembly lines.
Koglin et al. (2009)
5 more