› 2rpv

Solution NMR

Solution Structure of GB1 with LBT probe

Released:
DOI: 10.2210/pdb2rpv/pdb
PDB entry 2rpv coloured by chain, ensemble of 20 models, front view.
PDB entry 2rpv coloured by chain, ensemble of 20 models, side view.
PDB entry 2rpv coloured by chain, ensemble of 20 models, top view.
Source organism: Streptococcus sp. 'group G'
Primary publication:
Two-point anchoring of a lanthanide-binding peptide to a target protein enhances the paramagnetic anisotropic effect.
Saio T, Ogura K, Yokochi M, Kobashigawa Y, Inagaki F
J Biomol NMR 44 157-66 (2009)
PMID: 19468839

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139123 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein G Chain: A
Molecule details ›
Chain: A
Length: 75 amino acids
Theoretical weight: 8.21 KDa
Source organism: Streptococcus sp. 'group G'
Expression system: Escherichia coli
UniProt:
  • Canonical: P06654 (Residues: 223-282; Coverage: 15%)
Gene name: spg
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

1 bound ligand:
Ligand LA 1 x LA
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Protein NMR using paramagnetic ions.
Otting G. (2010)
10 more

4 mentions without citation

Expanding the utility of NMR restraints with paramagnetic compounds: background and practical aspects.
Koehler et al. (2011)
3 more