› 2tod

X-ray diffraction
2Å resolution

ORNITHINE DECARBOXYLASE FROM TRYPANOSOMA BRUCEI K69A MUTANT IN COMPLEX WITH ALPHA-DIFLUOROMETHYLORNITHINE

Released:
DOI: 10.2210/pdb2tod/pdb
PDB entry 2tod coloured by chain, front view.
PDB entry 2tod coloured by chain, side view.
PDB entry 2tod coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
X-ray structure of ornithine decarboxylase from Trypanosoma brucei: the native structure and the structure in complex with alpha-difluoromethylornithine.
Grishin NV, Osterman AL, Brooks HB, Phillips MA, Goldsmith EJ
Biochemistry 38 15174-84 (1999)
PMID: 10563800

Function and Biology Details

Reaction catalysed: 
L-ornithine = putrescine + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139767 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ornithine decarboxylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 425 amino acids
Theoretical weight: 47 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: P07805 (Residues: 1-423; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 4 x PLP
1 bound ligand:
Ligand DMO 4 x DMO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21
Unit cell:
a: 66.8Å b: 154.5Å c: 77.1Å
α: 90° β: 90.58° γ: 90°
R-values:
R R work R free
0.212 0.212 0.27
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Fold change in evolution of protein structures.
Grishin NV. (2001)
17 more

8 mentions without citation

State of the art in African trypanosome drug discovery.
Jacobs et al. (2011)
7 more