› 2uuq

X-ray diffraction
1.46Å resolution

Crystal structure of CYP130 from Mycobacterium tuberculosis in the ligand-free form

Released:
DOI: 10.2210/pdb2uuq/pdb
PDB entry 2uuq coloured by chain, front view.
PDB entry 2uuq coloured by chain, side view.
PDB entry 2uuq coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Mycobacterium tuberculosis CYP130: crystal structure, biophysical characterization, and interactions with antifungal azole drugs.
Ouellet H, Podust LM, de Montellano PR
J Biol Chem 283 5069-80 (2008)
PMID: 18089574

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161995 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 130 Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 45.84 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P9WPN5 (Residues: 1-405; Coverage: 100%)
Gene names: MTCY50.26, Rv1256c, cyp130
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand IPA 4 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID, ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 160.005Å b: 53.854Å c: 43.726Å
α: 90° β: 96.49° γ: 90°
R-values:
R R work R free
0.199 0.196 0.227
Expression system: Escherichia coli BL21

Quick links

Citations

8 review citations

Clinical and molecular pharmacology of etomidate.
Forman SA. (2011)
7 more

8 mentions without citation

Conformational plasticity and structure/function relationships in cytochromes P450.
Pochapsky et al. (2010)
7 more