2uv0

X-ray diffraction
1.8Å resolution

Structure of the P. aeruginosa LasR ligand-binding domain bound to its autoinducer

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-150367 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional activator protein LasR Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 175 amino acids
Theoretical weight: 19.69 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25084 (Residues: 1-173; Coverage: 72%)
Gene names: PA1430, lasR
Sequence domains: Autoinducer binding domain
Structure domains: Transcription factor LuxR-like, autoinducer-binding domain

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P21
Unit cell:
a: 53.82Å b: 85.33Å c: 75.51Å
α: 90° β: 95.82° γ: 90°
R-values:
R R work R free
0.211 0.209 0.254
Expression system: Escherichia coli BL21(DE3)