› 2uvf

X-ray diffraction
2.1Å resolution

Structure of Yersinia enterocolitica Family 28 Exopolygalacturonase in Complex with Digalaturonic Acid

Released:
DOI: 10.2210/pdb2uvf/pdb
Model geometry
Fit model/data
PDB entry 2uvf coloured by chain, front view.
PDB entry 2uvf coloured by chain, side view.
PDB entry 2uvf coloured by chain, top view.
Source organism: Yersinia enterocolitica
Primary publication:
The structural basis for exopolygalacturonase activity in a family 28 glycoside hydrolase.
Abbott DW, Boraston AB
J Mol Biol 368 1215-22 (2007)
PMID: 17397864

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130637 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Fibronectin type-III domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 608 amino acids
Theoretical weight: 66.73 KDa
Source organism: Yersinia enterocolitica
Expression system: Escherichia coli
UniProt:
  • Canonical: O68975 (Residues: 1-600; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: ADA
Carbohydrate polymer
4 bound ligands:
Ligand SO4 18 x SO4
Ligand NI 10 x NI
Ligand ACT 4 x ACT
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
Spacegroup: P21
Unit cell:
a: 91.015Å b: 79.659Å c: 98.493Å
α: 90° β: 103.9° γ: 90°
R-values:
R R work R free
0.234 0.232 0.284
Expression system: Escherichia coli

Quick links

Citations

2 review citations

A Profusion of Molecular Scissors for Pectins: Classification, Expression, and Functions of Plant Polygalacturonases.
Yang et al. (2018)
1 more

4 mentions without citation

Structural biology of pectin degradation by Enterobacteriaceae.
Abbott et al. (2008)
3 more