› 2uwm

X-ray diffraction
2.31Å resolution

C-TERMINAL DOMAIN(WH2-WH4) OF ELONGATION FACTOR SELB IN COMPLEX WITH SECIS RNA

Released:
DOI: 10.2210/pdb2uwm/pdb
PDB entry 2uwm coloured by chain, front view.
PDB entry 2uwm coloured by chain, side view.
PDB entry 2uwm coloured by chain, top view.
Source organism: Moorella thermoacetica
Primary publication:
Structural basis for dynamic interdomain movement and RNA recognition of the selenocysteine-specific elongation factor SelB.
Ose T, Soler N, Rasubala L, Kuroki K, Kohda D, Fourmy D, Yoshizawa S, Maenaka K
Structure 15 577-86 (2007)
PMID: 17502103

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173890 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Selenocysteine-specific elongation factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 29.69 KDa
Source organism: Moorella thermoacetica
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q2RFK4 (Residues: 377-634; Coverage: 41%)
Gene name: Moth_2503
Sequence domains:
Structure domains:
5'-R(*GP*GP*CP*GP*UP*UP*GP*CP*CP*GP *GP*UP*CP*UP*GP*GP*CP*AP*AP*CP*GP*CP*C)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 23 nucleotides
Theoretical weight: 7.39 KDa
Source organism: Moorella thermoacetica
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: C2
Unit cell:
a: 160.074Å b: 119.843Å c: 50.461Å
α: 90° β: 100.05° γ: 90°
R-values:
R R work R free
0.204 0.2 0.238
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

2 review citations

The unique tRNASec and its role in selenocysteine biosynthesis.
Serrão et al. (2018)
1 more

4 mentions without citation

Towards deciphering the principles underlying an mRNA recognition code.
Serganov et al. (2008)
3 more