› 2uxf

X-ray diffraction
2Å resolution

Pseudoazurin with engineered amicyanin ligand loop, oxidized form, pH 5.5

Released:
DOI: 10.2210/pdb2uxf/pdb
PDB entry 2uxf coloured by chain, front view.
PDB entry 2uxf coloured by chain, side view.
PDB entry 2uxf coloured by chain, top view.
Source organism: Achromobacter cycloclastes
Primary publication:
Influence of loop shortening on the metal binding site of cupredoxin pseudoazurin.
Velarde M, Huber R, Yanagisawa S, Dennison C, Messerschmidt A
Biochemistry 46 9981-91 (2007)
PMID: 17685636

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148646 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pseudoazurin Chain: A
Molecule details ›
Chain: A
Length: 122 amino acids
Theoretical weight: 12.87 KDa
Source organism: Achromobacter cycloclastes
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P19567 (Residues: 29-152; Coverage: 98%)
Gene name: bcp
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

3 bound ligands:
Ligand CU 1 x CU
Ligand GOL 1 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: R32
Unit cell:
a: 105.572Å b: 105.572Å c: 57.286Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.164 0.204
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

The role of ligand-containing loops at copper sites in proteins.
Dennison C. (2008)
8 other articles