2uyn

X-ray diffraction
1.6Å resolution

Crystal structure of E. coli TdcF with bound 2-ketobutyrate

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-142842 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Putative reactive intermediate deaminase TdcF Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.11 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AGL2 (Residues: 1-129; Coverage: 100%)
Gene names: JW5521, b3113, tdcF, yhaR
Sequence domains: Endoribonuclease L-PSP
Structure domains: RutC-like
Putative reactive intermediate deaminase TdcF Chains: B, C
Molecule details ›
Chains: B, C
Length: 129 amino acids
Theoretical weight: 14.07 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AGL2 (Residues: 1-129; Coverage: 100%)
Gene names: JW5521, b3113, tdcF, yhaR
Sequence domains: Endoribonuclease L-PSP
Structure domains: RutC-like

Ligands and Environments

1 bound ligand:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 72.581Å b: 85.901Å c: 62.661Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.204
Expression system: Escherichia coli BL21(DE3)