› 2uyq

X-ray diffraction
1.8Å resolution

Crystal structure of ML2640c from Mycobacterium leprae in complex with S-adenosylmethionine

Released:
DOI: 10.2210/pdb2uyq/pdb
PDB entry 2uyq coloured by chain, front view.
PDB entry 2uyq coloured by chain, side view.
PDB entry 2uyq coloured by chain, top view.
Source organism: Mycobacterium leprae
Primary publication:
The crystal structure of M. leprae ML2640c defines a large family of putative S-adenosylmethionine-dependent methyltransferases in mycobacteria.
Graña M, Haouz A, Buschiazzo A, Miras I, Wehenkel A, Bondet V, Shepard W, Schaeffer F, Cole ST, Alzari PM
Protein Sci 16 1896-904 (2007)
PMID: 17660248

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative S-adenosyl-L-methionine-dependent methyltransferase ML2640 Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 34.49 KDa
Source organism: Mycobacterium leprae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9CCZ4 (Residues: 1-310; Coverage: 100%)
Gene name: ML2640
Sequence domains: Leucine carboxyl methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand SAM 1 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P65
Unit cell:
a: 75.826Å b: 75.826Å c: 105.655Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.19 0.231
Expression system: Escherichia coli BL21

Quick links

Citations

8 citation in other articles

TubercuList--10 years after.
Lew et al. (2011)
7 more

4 mentions without citation

Structure-Guided Computational Approaches to Unravel Druggable Proteomic Landscape of Mycobacterium leprae.
Vedithi et al. (2021)
3 more