› 2uz0

X-ray diffraction
1.7Å resolution

The Crystal crystal structure of the estA protein, a virulence factor estA protein from Streptococcus pneumonia

Released:
DOI: 10.2210/pdb2uz0/pdb
PDB entry 2uz0 coloured by chain, front view.
PDB entry 2uz0 coloured by chain, side view.
PDB entry 2uz0 coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
The crystal structure of the estA protein, a virulence factor from Streptococcus pneumoniae.
Kim MH, Kang BS, Kim S, Kim KJ, Lee CH, Oh BC, Park SC, Oh TK
Proteins 70 578-83 (2008)
PMID: 17932928

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101415 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tributyrin esterase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 263 amino acids
Theoretical weight: 30.6 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2UNZ8 (Residues: 2-259; Coverage: 100%)
Gene names: SP_0614, estA
Sequence domains: Putative esterase
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand GOL 6 x GOL
Ligand EDO 4 x EDO
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P21
Unit cell:
a: 71.695Å b: 72.128Å c: 101.827Å
α: 90° β: 94.31° γ: 90°
R-values:
R R work R free
0.184 0.183 0.213
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Glycan-metabolizing enzymes in microbe-host interactions: the Streptococcus pneumoniae paradigm.
Hobbs et al. (2018)
1 more

6 mentions without citation

Thioesterases: a new perspective based on their primary and tertiary structures.
Cantu et al. (2010)
5 more