› 2v1b

X-ray diffraction
1.55Å resolution

N- and C-terminal helices of oat LOV2 (404-546) are involved in light-induced signal transduction (room temperature (293K) light structure of LOV2 (404-546))

Released:
DOI: 10.2210/pdb2v1b/pdb
PDB entry 2v1b coloured by chain, front view.
PDB entry 2v1b coloured by chain, side view.
PDB entry 2v1b coloured by chain, top view.
Source organism: Avena sativa
Primary publication:
N- and C-terminal flanking regions modulate light-induced signal transduction in the LOV2 domain of the blue light sensor phototropin 1 from Avena sativa.
Halavaty AS, Moffat K
Biochemistry 46 14001-9 (2007)
PMID: 18001137

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129262 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
non-specific serine/threonine protein kinase Chain: A
Molecule details ›
Chain: A
Length: 144 amino acids
Theoretical weight: 16.67 KDa
Source organism: Avena sativa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O49003 (Residues: 404-546; Coverage: 16%)
Gene name: NPH1-1
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 35.919Å b: 57.169Å c: 67.228Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

39 review citations

Structure and signaling mechanism of Per-ARNT-Sim domains.
Möglich et al. (2009)
38 more

10 mentions without citation

Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators.
Lu et al. (2020)
9 more