› 2v1l

X-ray diffraction
2.13Å resolution

Structure of the conserved hypothetical protein VC1805 from pathogenicity island VPI-2 of Vibrio cholerae O1 biovar eltor str. N16961 shares structural homology with the human P32 protein

Released:
DOI: 10.2210/pdb2v1l/pdb
PDB entry 2v1l coloured by chain, front view.
PDB entry 2v1l coloured by chain, side view.
PDB entry 2v1l coloured by chain, top view.
Source organism: Vibrio cholerae O1
Primary publication:
Crystal structure of VC1805, a conserved hypothetical protein from a Vibrio cholerae pathogenicity island, reveals homology to human p32.
Sheikh MA, Potter JA, Johnson KA, Sim RB, Boyd EF, Taylor GL
Proteins 71 1563-71 (2008)
PMID: 18300248

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192053 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF2787 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.85 KDa
Source organism: Vibrio cholerae O1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KR41 (Residues: 1-148; Coverage: 100%)
Gene name: VC_1805
Sequence domains: Protein of unknown function (DUF2787)
Structure domains: Protein of unknown function DUF2787

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P3121
Unit cell:
a: 78.312Å b: 78.312Å c: 42.288Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.235 0.316
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

A simple and efficient expression and purification system using two newly constructed vectors.
Liu et al. (2009)
2 more

1 mention without citation

The Scottish Structural Proteomics Facility: targets, methods and outputs.
Oke et al. (2010)