› 2v1w

X-ray diffraction
1.9Å resolution

Crystal structure of human LIM protein RIL (PDLIM4) PDZ domain bound to the C-terminal peptide of human alpha-actinin-1

Released:
DOI: 10.2210/pdb2v1w/pdb
PDB entry 2v1w coloured by chain, front view.
PDB entry 2v1w coloured by chain, side view.
PDB entry 2v1w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Unusual binding interactions in PDZ domain crystal structures help explain binding mechanisms.
Elkins JM, Gileadi C, Shrestha L, Phillips C, Wang J, Muniz JR, Doyle DA
Protein Sci 19 731-41 (2010)
PMID: 20120020

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156197 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PDZ and LIM domain protein 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 90 amino acids
Theoretical weight: 9.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50479 (Residues: 1-87; Coverage: 26%)
Gene names: PDLIM4, RIL
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

3 bound ligands:
Ligand EDO 1 x EDO
Ligand MG 1 x MG
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P4212
Unit cell:
a: 87.029Å b: 87.029Å c: 53.917Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.211
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

PDZ Domains as Drug Targets.
Christensen et al. (2019)
4 more

1 mention without citation

Unusual binding interactions in PDZ domain crystal structures help explain binding mechanisms.
Elkins et al. (2010)