› 2v3a

X-ray diffraction
2.4Å resolution

Crystal structure of rubredoxin reductase from Pseudomonas aeruginosa.

Released:
DOI: 10.2210/pdb2v3a/pdb
PDB entry 2v3a coloured by chain, front view.
PDB entry 2v3a coloured by chain, side view.
PDB entry 2v3a coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Crystal structure of the electron transfer complex rubredoxin rubredoxin reductase of Pseudomonas aeruginosa.
Hagelueken G, Wiehlmann L, Adams TM, Kolmar H, Heinz DW, Tümmler B, Schubert WD
Proc Natl Acad Sci U S A 104 12276-81 (2007)
PMID: 17636129

Function and Biology Details

Reaction catalysed: 
2 reduced rubredoxin + NAD(+) + H(+) = 2 oxidized rubredoxin + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190929 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rubredoxin-NAD(+) reductase Chain: A
Molecule details ›
Chain: A
Length: 384 amino acids
Theoretical weight: 40.66 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HTK9 (Residues: 1-384; Coverage: 100%)
Gene names: PA5349, alkT
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
2 bound ligands:
Ligand PEG 6 x PEG
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P6122
Unit cell:
a: 119.602Å b: 119.602Å c: 158.064Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.166 0.202
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
2 more

2 mentions without citation

Crystal structure of the electron transfer complex rubredoxin rubredoxin reductase of Pseudomonas aeruginosa.
Hagelueken et al. (2007)
1 more