› 2v43

X-ray diffraction
2.37Å resolution

Crystal structure of RseB: a sensor for periplasmic stress response in E. coli

Released:
DOI: 10.2210/pdb2v43/pdb
PDB entry 2v43 coloured by chain, front view.
PDB entry 2v43 coloured by chain, side view.
PDB entry 2v43 coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
The structure of RseB: a sensor in periplasmic stress response of E. coli.
Wollmann P, Zeth K
J Mol Biol 372 927-941 (2007)
PMID: 17692869

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142767 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sigma-E factor regulatory protein RseB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 303 amino acids
Theoretical weight: 34.36 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFX9 (Residues: 23-318; Coverage: 100%)
Gene names: JW2555, b2571, rseB
Sequence domains:
Structure domains: Lipoprotein localisation LolA/LolB/LppX

Ligands and Environments

1 bound ligand:
Ligand CYS 4 x CYS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 98.6Å b: 200.7Å c: 109.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.228 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Lipoprotein sorting in bacteria.
Okuda et al. (2011)
9 more

1 mention without citation

Structural basis for the negative regulation of bacterial stress response by RseB.
Kim et al. (2010)