› 2v50

X-ray diffraction
3Å resolution

The Missing Part of the Bacterial MexAB-OprM System: Structural determination of the Multidrug Exporter MexB

Released:
DOI: 10.2210/pdb2v50/pdb
PDB entry 2v50 coloured by chain, front view.
PDB entry 2v50 coloured by chain, side view.
PDB entry 2v50 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Crystal structure of the multidrug exporter MexB from Pseudomonas aeruginosa.
Sennhauser G, Bukowska MA, Briand C, Grütter MG
J Mol Biol 389 134-45 (2009)
PMID: 19361527

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug resistance protein MexB Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 1052 amino acids
Theoretical weight: 113.71 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52002 (Residues: 1-1046; Coverage: 100%)
Gene names: PA0426, mexB
Sequence domains: AcrB/AcrD/AcrF family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand LMT 8 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P1
Unit cell:
a: 125.05Å b: 134.58Å c: 151.02Å
α: 86.99° β: 69.7° γ: 88.16°
R-values:
R R work R free
0.243 0.242 0.287
Expression system: Escherichia coli BL21(DE3)

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Citations

38 review citations

The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.
Li et al. (2015)
37 more

30 mentions without citation

Carbapenems: past, present, and future.
Papp-Wallace et al. (2011)
29 more