› 2v8h

X-ray diffraction
2Å resolution

Crystal structure of mutant E159A of beta-alanine synthase from Saccharomyces kluyveri in complex with its substrate N-carbamyl-beta- alanine

Released:
DOI: 10.2210/pdb2v8h/pdb
Model geometry
Fit model/data
PDB entry 2v8h coloured by chain, front view.
PDB entry 2v8h coloured by chain, side view.
PDB entry 2v8h coloured by chain, top view.
Source organism: Lachancea kluyveri
Primary publication:
Crystal structures of yeast beta-alanine synthase complexes reveal the mode of substrate binding and large scale domain closure movements.
Lundgren S, Andersen B, Piskur J, Dobritzsch D
J Biol Chem 282 36037-47 (2007)
PMID: 17916556

Function and Biology Details

Reaction catalysed: 
3-ureidopropanoate + H(2)O = beta-alanine + CO(2) + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188776 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase M20 dimerisation domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 474 amino acids
Theoretical weight: 51.95 KDa
Source organism: Lachancea kluyveri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96W94 (Residues: 2-455; Coverage: 100%)
Gene name: PYD3
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 8 x ZN
Ligand URP 4 x URP
Ligand BCN 4 x BCN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P21
Unit cell:
a: 49.78Å b: 218.3Å c: 81.58Å
α: 90° β: 92.19° γ: 90°
R-values:
R R work R free
0.18 0.178 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 citation in other articles

The crystal structure of beta-alanine synthase from Drosophila melanogaster reveals a homooctameric helical turn-like assembly.
Lundgren et al. (2008)
8 more

3 mentions without citation

Structural and functional analyses reveal that Staphylococcus aureus antibiotic resistance factor HmrA is a zinc-dependent endopeptidase.
Botelho et al. (2011)
2 more