› 2v91

X-ray diffraction
3.01Å resolution

STRUCTURE OF STRICTOSIDINE SYNTHASE IN COMPLEX WITH STRICTOSIDINE

Released:
DOI: 10.2210/pdb2v91/pdb
PDB entry 2v91 coloured by chain, front view.
PDB entry 2v91 coloured by chain, side view.
PDB entry 2v91 coloured by chain, top view.
Source organism: Rauvolfia serpentina
Primary publication:
Structure-based engineering of strictosidine synthase: auxiliary for alkaloid libraries.
Loris EA, Panjikar S, Ruppert M, Barleben L, Unger M, Schübel H, Stöckigt J
Chem Biol 14 979-85 (2007)
PMID: 17884630

Function and Biology Details

Reaction catalysed: 
3-alpha-(S)-strictosidine + H(2)O = tryptamine + secologanin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159353 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Strictosidine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 302 amino acids
Theoretical weight: 33.69 KDa
Source organism: Rauvolfia serpentina
Expression system: Escherichia coli
UniProt:
  • Canonical: P68175 (Residues: 32-333; Coverage: 94%)
Gene name: STR1
Sequence domains: Strictosidine synthase
Structure domains: TolB, C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand S55 2 x S55
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: R3
Unit cell:
a: 150.177Å b: 150.177Å c: 121.707Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.192 0.235
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Alkaloid biosynthesis: metabolism and trafficking.
Ziegler et al. (2008)
15 more

4 mentions without citation

The evolution of function in strictosidine synthase-like proteins.
Hicks et al. (2011)
3 more