› 2va8

X-ray diffraction
2.3Å resolution

DNA Repair Helicase Hel308

Released:
DOI: 10.2210/pdb2va8/pdb
PDB entry 2va8 coloured by chain, front view.
PDB entry 2va8 coloured by chain, side view.
PDB entry 2va8 coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Structure of the DNA repair helicase hel308 reveals DNA binding and autoinhibitory domains.
Richards JD, Johnson KA, Liu H, McRobbie AM, McMahon S, Oke M, Carter L, Naismith JH, White MF
J Biol Chem 283 5118-26 (2008)
PMID: 18056710

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163264 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SKI2-TYPE HELICASE Chains: A, B
Molecule details ›
Chains: A, B
Length: 715 amino acids
Theoretical weight: 81.58 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1, ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 61.657Å b: 138.083Å c: 107.551Å
α: 90° β: 94.65° γ: 90°
R-values:
R R work R free
0.214 0.212 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Non-hexameric DNA helicases and translocases: mechanisms and regulation.
Lohman et al. (2008)
18 more

7 mentions without citation

Ski2-like RNA helicase structures: common themes and complex assemblies.
Johnson et al. (2013)
6 more