› 2vbj

X-ray diffraction
1.95Å resolution

Molecular basis of human XPC gene recognition and cleavage by engineered homing endonuclease heterodimers

Released:
DOI: 10.2210/pdb2vbj/pdb
PDB entry 2vbj coloured by chain, front view.
PDB entry 2vbj coloured by chain, side view.
PDB entry 2vbj coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Molecular basis of xeroderma pigmentosum group C DNA recognition by engineered meganucleases.
Redondo P, Prieto J, Muñoz IG, Alibés A, Stricher F, Serrano L, Cabaniols JP, Daboussi F, Arnould S, Perez C, Duchateau P, Pâques F, Blanco FJ, Montoya G
Nature 456 107-11 (2008)
PMID: 18987743

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115870 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA endonuclease I-CreI Chain: A
Molecule details ›
Chain: A
Length: 152 amino acids
Theoretical weight: 17.57 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P05725 (Residues: 2-153; Coverage: 93%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
DNA endonuclease I-CreI Chain: B
Molecule details ›
Chain: B
Length: 152 amino acids
Theoretical weight: 17.45 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P05725 (Residues: 2-153; Coverage: 93%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
5'-D(*TP*CP*TP*GP*CP*CP*TP*TP*TP*TP *TP*TP*GP*AP*AP*GP*GP*AP*TP*CP*CP*TP*AP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 24 nucleotides
Theoretical weight: 7.33 KDa
5'-D(*TP*TP*AP*GP*GP*AP*TP*CP*CP*TP *TP*CP*AP*AP*AP*AP*AP*AP*GP*GP*CP*AP*GP*A)-3' Chain: E
Molecule details ›
Chain: E
Length: 24 nucleotides
Theoretical weight: 7.41 KDa

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 44.552Å b: 68.7Å c: 88.731Å
α: 90° β: 95.36° γ: 90°
R-values:
R R work R free
0.15 0.147 0.216
Expression system: Escherichia coli

Quick links

Citations

37 review citations

Genome-editing Technologies for Gene and Cell Therapy.
Maeder et al. (2016)
36 more

3 mentions without citation

Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus.
Muñoz et al. (2011)
2 more