› 2vbs

X-ray diffraction
3Å resolution

Riboflavin kinase Mj0056 from Methanocaldococcus jannaschii in complex with PO4

Released:
DOI: 10.2210/pdb2vbs/pdb
PDB entry 2vbs coloured by chain, front view.
PDB entry 2vbs coloured by chain, side view.
PDB entry 2vbs coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
A CTP-dependent archaeal riboflavin kinase forms a bridge in the evolution of cradle-loop barrels.
Ammelburg M, Hartmann MD, Djuranovic S, Alva V, Koretke KK, Martin J, Sauer G, Truffault V, Zeth K, Lupas AN, Coles M
Structure 15 1577-90 (2007)
PMID: 18073108

Function and Biology Details

Reaction catalysed: 
CTP + riboflavin = CDP + FMN
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178777 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Riboflavin kinase Chain: A
Molecule details ›
Chain: A
Length: 136 amino acids
Theoretical weight: 15.71 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60365 (Residues: 1-132; Coverage: 100%)
Gene names: MJ0056, ribK
Sequence domains: Domain of unknown function DUF120
Structure domains: Riboflavin kinase-like

Ligands and Environments

3 bound ligands:
Ligand PO4 1 x PO4
Ligand ZN 1 x ZN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P43212
Unit cell:
a: 77.7Å b: 77.7Å c: 107.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.23 0.282
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers.
Abbas et al. (2011)
1 more

2 mentions without citation

PDBsum: Structural summaries of PDB entries.
Laskowski et al. (2018)
1 more