› 2vd9

X-ray diffraction
2.1Å resolution

The crystal structure of alanine racemase from Bacillus anthracis (BA0252) with bound L-Ala-P

Released:
DOI: 10.2210/pdb2vd9/pdb
PDB entry 2vd9 coloured by chain, front view.
PDB entry 2vd9 coloured by chain, side view.
PDB entry 2vd9 coloured by chain, top view.
Source organism: Bacillus anthracis str. Ames
Primary publication:
Structures of an alanine racemase from Bacillus anthracis (BA0252) in the presence and absence of (R)-1-aminoethylphosphonic acid (L-Ala-P).
Au K, Ren J, Walter TS, Harlos K, Nettleship JE, Owens RJ, Stuart DI, Esnouf RM
Acta Crystallogr Sect F Struct Biol Cryst Commun 64 327-33 (2008)
PMID: 18453697

Function and Biology Details

Reaction catalysed: 
L-alanine = D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine racemase Chains: A, B
Molecule details ›
Chains: A, B
Length: 391 amino acids
Theoretical weight: 44.35 KDa
Source organism: Bacillus anthracis str. Ames
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A6L7HC45 (Residues: 1-389; Coverage: 100%)
Gene names: GBAA_0252, dal1
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 3 x MG
Ligand CL 3 x CL
Ligand IN5 2 x IN5
Ligand EPC 2 x EPC
1 modified residue:
Ligand MLY 36 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 59.692Å b: 96.504Å c: 140.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

The crystal structure of alanine racemase from Streptococcus pneumoniae, a target for structure-based drug design.
Im et al. (2011)
3 more

2 mentions without citation

Structures of an alanine racemase from Bacillus anthracis (BA0252) in the presence and absence of (R)-1-aminoethylphosphonic acid (L-Ala-P).
Au et al. (2008)
1 more