› 2vdd

X-ray diffraction
3.3Å resolution

Crystal Structure of the Open State of TolC Outer Membrane Component of Mutlidrug Efflux Pumps

Released:
DOI: 10.2210/pdb2vdd/pdb
PDB entry 2vdd coloured by chain, front view.
PDB entry 2vdd coloured by chain, side view.
PDB entry 2vdd coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Assembly and channel opening in a bacterial drug efflux machine.
Bavro VN, Pietras Z, Furnham N, Pérez-Cano L, Fernández-Recio J, Pei XY, Misra R, Luisi B
Mol Cell 30 114-21 (2008)
PMID: 18406332

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136391 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane protein TolC Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 460 amino acids
Theoretical weight: 50.62 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P02930 (Residues: 1-450; Coverage: 91%)
Gene names: JW5503, b3035, colE1-i, mtcB, mukA, refI, toc, tolC, weeA
Sequence domains: Outer membrane efflux protein
Structure domains: Outer membrane efflux proteins (OEP)

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand K 3 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 122.485Å b: 70.966Å c: 219.951Å
α: 90° β: 100.61° γ: 90°
R-values:
R R work R free
0.239 0.236 0.295
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
26 more

6 mentions without citation

The bacterial outer membrane β-barrel assembly machinery.
Kim et al. (2012)
5 more