› 2vdy

X-ray diffraction
2.3Å resolution

Crystal structure of the reactive loop cleaved Corticosteroid Binding Globulin complexed with Cortisol

Released:
DOI: 10.2210/pdb2vdy/pdb
PDB entry 2vdy coloured by chain, front view.
PDB entry 2vdy coloured by chain, side view.
PDB entry 2vdy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The S-to-R transition of corticosteroid-binding globulin and the mechanism of hormone release.
Zhou A, Wei Z, Stanley PL, Read RJ, Stein PE, Carrell RW
J Mol Biol 380 244-51 (2008)
PMID: 18513745

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139996 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Corticosteroid-binding globulin Chains: A, B
Molecule details ›
Chains: A, B
Length: 373 amino acids
Theoretical weight: 41.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08185 (Residues: 33-405; Coverage: 97%)
Gene names: CBG, SERPINA6
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand HCY 2 x HCY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P1
Unit cell:
a: 38.673Å b: 39.757Å c: 111.586Å
α: 83.09° β: 85.7° γ: 69.42°
R-values:
R R work R free
0.213 0.21 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Conformational pathology of the serpins: themes, variations, and therapeutic strategies.
Gooptu et al. (2009)
11 more

8 mentions without citation

How changes in affinity of corticosteroid-binding globulin modulate free cortisol concentration.
Chan et al. (2013)
7 more