› 2ve3

X-ray diffraction
2.1Å resolution

Retinoic acid bound cyanobacterial CYP120A1

Released:
DOI: 10.2210/pdb2ve3/pdb
PDB entry 2ve3 coloured by chain, front view.
PDB entry 2ve3 coloured by chain, side view.
PDB entry 2ve3 coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Crystal structures of substrate-free and retinoic acid-bound cyanobacterial cytochrome P450 CYP120A1.
Kühnel K, Ke N, Cryle MJ, Sligar SG, Schuler MA, Schlichting I
Biochemistry 47 6552-9 (2008)
PMID: 18512957

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176837 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative cytochrome P450 120 Chains: A, B
Molecule details ›
Chains: A, B
Length: 444 amino acids
Theoretical weight: 50.63 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q59990 (Residues: 1-444; Coverage: 100%)
Gene names: cyp, cyp120, slr0574
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand REA 2 x REA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 141.46Å b: 132.97Å c: 67.42Å
α: 90° β: 114.5° γ: 90°
R-values:
R R work R free
0.225 0.223 0.267
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Exploiting cyanobacterial P450 pathways.
Robert et al. (2010)
1 more

17 mentions without citation

Conformational plasticity and structure/function relationships in cytochromes P450.
Pochapsky et al. (2010)
16 more