› 2ve4

X-ray diffraction
2.4Å resolution

Substrate free cyanobacterial CYP120A1

Released:
DOI: 10.2210/pdb2ve4/pdb
PDB entry 2ve4 coloured by chain, front view.
PDB entry 2ve4 coloured by chain, side view.
PDB entry 2ve4 coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Crystal structures of substrate-free and retinoic acid-bound cyanobacterial cytochrome P450 CYP120A1.
Kühnel K, Ke N, Cryle MJ, Sligar SG, Schuler MA, Schlichting I
Biochemistry 47 6552-9 (2008)
PMID: 18512957

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176837 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative cytochrome P450 120 Chains: A, B
Molecule details ›
Chains: A, B
Length: 444 amino acids
Theoretical weight: 50.63 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q59990 (Residues: 1-444; Coverage: 100%)
Gene names: cyp, cyp120, slr0574
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

1 bound ligand:
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 153.64Å b: 122.17Å c: 64.92Å
α: 90° β: 115° γ: 90°
R-values:
R R work R free
0.234 0.23 0.304
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Biochemical and physiological importance of the CYP26 retinoic acid hydroxylases.
Isoherranen et al. (2019)
2 more

1 mention without citation

Crystal structures of Trypanosoma brucei sterol 14alpha-demethylase and implications for selective treatment of human infections.
Lepesheva et al. (2010)