› 2vii

X-ray diffraction
2.85Å resolution

PspF1-275-Mg-AMP

Released:
DOI: 10.2210/pdb2vii/pdb
PDB entry 2vii coloured by chain, front view.
PDB entry 2vii coloured by chain, side view.
PDB entry 2vii coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Trapping of a transcription complex using a new nucleotide analogue: AMP aluminium fluoride.
Joly N, Rappas M, Buck M, Zhang X
J Mol Biol 375 1206-11 (2008)
PMID: 18082766

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Psp operon transcriptional activator Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 29.39 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37344 (Residues: 1-259; Coverage: 80%)
Gene names: JW1296, b1303, pspF, ycjB
Sequence domains: Sigma-54 interaction domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand AMP 1 x AMP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P65
Unit cell:
a: 113.607Å b: 113.607Å c: 39.358Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.186 0.241
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.
Bush et al. (2012)
3 more

3 mentions without citation

Regulation of microglial development: a novel role for thyroid hormone.
Lima et al. (2001)
2 more