› 2vji

X-ray diffraction
1.38Å resolution

Tailspike protein of E.coli bacteriophage HK620

Released:
DOI: 10.2210/pdb2vji/pdb
PDB entry 2vji coloured by chain, front view.
PDB entry 2vji coloured by chain, side view.
PDB entry 2vji coloured by chain, top view.
Source organism: Enterobacteria phage HK620
Primary publication:
Crystal structure of Escherichia coli phage HK620 tailspike: podoviral tailspike endoglycosidase modules are evolutionarily related.
Barbirz S, Müller JJ, Uetrecht C, Clark AJ, Heinemann U, Seckler R
Mol Microbiol 69 303-16 (2008)
PMID: 18547389

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189615 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriophage P22 tailspike N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 600 amino acids
Theoretical weight: 64.71 KDa
Source organism: Enterobacteria phage HK620
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AYY6 (Residues: 111-710; Coverage: 85%)
Gene name: 9
Sequence domains: Phage spike trimer
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand EDO 1 x EDO
Ligand PG0 1 x PG0
Ligand PO4 1 x PO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P321
Unit cell:
a: 74.216Å b: 74.216Å c: 175.16Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.151 0.15 0.18
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

A common evolutionary origin for tailed-bacteriophage functional modules and bacterial machineries.
Veesler et al. (2011)
12 more

3 mentions without citation

Flexible Backbone Assembly and Refinement of Symmetrical Homomeric Complexes.
Roy Burman et al. (2019)
2 more