› 2vjj

X-ray diffraction
1.59Å resolution

TAILSPIKE PROTEIN OF E.COLI BACTERIOPHAGE HK620 IN COMPLEX WITH HEXASACCHARIDE

Released:
DOI: 10.2210/pdb2vjj/pdb
PDB entry 2vjj coloured by chain, front view.
PDB entry 2vjj coloured by chain, side view.
PDB entry 2vjj coloured by chain, top view.
Source organism: Enterobacteria phage HK620
Primary publication:
Crystal structure of Escherichia coli phage HK620 tailspike: podoviral tailspike endoglycosidase modules are evolutionarily related.
Barbirz S, Müller JJ, Uetrecht C, Clark AJ, Heinemann U, Seckler R
Mol Microbiol 69 303-16 (2008)
PMID: 18547389

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189615 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Bacteriophage P22 tailspike N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 600 amino acids
Theoretical weight: 64.71 KDa
Source organism: Enterobacteria phage HK620
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9AYY6 (Residues: 111-710; Coverage: 85%)
Gene name: 9
Sequence domains: Phage spike trimer
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GAL, GLC, RAM
Carbohydrate polymer
4 bound ligands:
Ligand CA 1 x CA
Ligand CL 1 x CL
Ligand K 1 x K
Ligand GOL 7 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P321
Unit cell:
a: 73.91Å b: 73.91Å c: 174.59Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.134 0.133 0.165
Expression system: Escherichia coli BL21

Quick links

Citations

13 review citations

Bacteriophage-encoded depolymerases: their diversity and biotechnological applications.
Pires et al. (2016)
12 more

3 mentions without citation

Crystal structure of ORF210 from E. coli O157:H1 phage CBA120 (TSP1), a putative tailspike protein.
Chen et al. (2014)
2 more