› 2vjo

X-ray diffraction
2.2Å resolution

Formyl-CoA transferase mutant variant Q17A with aspartyl-CoA thioester intermediates and oxalate

Released:
DOI: 10.2210/pdb2vjo/pdb
PDB entry 2vjo coloured by chain, front view.
PDB entry 2vjo coloured by chain, side view.
PDB entry 2vjo coloured by chain, top view.
Source organism: Oxalobacter formigenes
Primary publication:
Reinvestigation of the catalytic mechanism of formyl-CoA transferase, a class III CoA-transferase.
Berthold CL, Toyota CG, Richards NG, Lindqvist Y
J Biol Chem 283 6519-29 (2008)
PMID: 18162462

Function and Biology Details

Reaction catalysed: 
Formyl-CoA + oxalate = formate + oxalyl-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126525 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formyl-CoA:oxalate CoA-transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 428 amino acids
Theoretical weight: 47.31 KDa
Source organism: Oxalobacter formigenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O06644 (Residues: 1-428; Coverage: 100%)
Gene name: frc
Sequence domains: CoA-transferase family III
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand COA 2 x COA
Ligand CL 2 x CL
Ligand OXL 1 x OXL
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: I4
Unit cell:
a: 153.593Å b: 153.593Å c: 98.055Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Biotechnological potential of the ethylmalonyl-CoA pathway.
Alber BE. (2011)
20 other articles

2 mentions without citation

Templated chemistry for bioorganic synthesis and chemical biology.
Seitz O. (2019)
1 more