› 2vk9

X-ray diffraction
2.85Å resolution

CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF ALPHA-TOXIN FROM CLOSTRIDIUM NOVYI

Released:
DOI: 10.2210/pdb2vk9/pdb
PDB entry 2vk9 coloured by chain, front view.
PDB entry 2vk9 coloured by chain, side view.
PDB entry 2vk9 coloured by chain, top view.
Source organism: Clostridium novyi
Primary publication:
Conformational changes and reaction of clostridial glycosylating toxins.
Ziegler MO, Jank T, Aktories K, Schulz GE
J Mol Biol 377 1346-56 (2008)
PMID: 18325534

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175103 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylglucosaminyltransferase TcdA Chain: A
Molecule details ›
Chain: A
Length: 551 amino acids
Theoretical weight: 64.06 KDa
Source organism: Clostridium novyi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46149 (Residues: 1-551; Coverage: 25%)
Gene names: tcdA, toxA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P61
Unit cell:
a: 154.612Å b: 154.612Å c: 77.605Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.224 0.257
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Structure and function of MARTX toxins and other large repetitive RTX proteins.
Satchell KJ. (2011)
8 more

9 mentions without citation

Toward a structural understanding of Clostridium difficile toxins A and B.
Pruitt et al. (2012)
8 more