› 2vkh

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF LETHAL TOXIN FROM CLOSTRIDIUM SORDELLII IN COMPLEX WITH UDP-GLC AND CALCIUM ION

Released:
DOI: 10.2210/pdb2vkh/pdb
PDB entry 2vkh coloured by chain, front view.
PDB entry 2vkh coloured by chain, side view.
PDB entry 2vkh coloured by chain, top view.
Source organism: Paeniclostridium sordellii
Primary publication:
Conformational changes and reaction of clostridial glycosylating toxins.
Ziegler MO, Jank T, Aktories K, Schulz GE
J Mol Biol 377 1346-56 (2008)
PMID: 18325534

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175109 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucosyltransferase TcsL Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 546 amino acids
Theoretical weight: 63.61 KDa
Source organism: Paeniclostridium sordellii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46342 (Residues: 1-546; Coverage: 23%)
Gene name: tcsL
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand UPG 3 x UPG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 57.17Å b: 188.59Å c: 203.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.235 0.273
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Clostridium difficile Toxin Biology.
Aktories et al. (2017)
9 more

4 mentions without citation

Toward a structural understanding of Clostridium difficile toxins A and B.
Pruitt et al. (2012)
3 more