› 2vks

X-ray diffraction
2.1Å resolution

Crystal structure of GAF-B domain of DevS from Mycobacterium smegmatis

Released:
DOI: 10.2210/pdb2vks/pdb
PDB entry 2vks coloured by chain, front view.
PDB entry 2vks coloured by chain, side view.
PDB entry 2vks coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis
Primary publication:
O2- and NO-sensing mechanism through the DevSR two-component system in Mycobacterium smegmatis.
Lee JM, Cho HY, Cho HJ, Ko IJ, Park SW, Baik HS, Oh JH, Eom CY, Kim YM, Kang BS, Oh JI
J Bacteriol 190 6795-804 (2008)
PMID: 18708494

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106436 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GAF family protein Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 15.22 KDa
Source organism: Mycolicibacterium smegmatis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0R2U9 (Residues: 232-380; Coverage: 26%)
Gene name: MSMEG_5241
Sequence domains: GAF domain
Structure domains: Beta-Lactamase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6C1
Spacegroup: P61
Unit cell:
a: 73.229Å b: 73.229Å c: 48.837Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.199 0.215
Expression system: Escherichia coli BL21

Quick links

Citations

10 review citations

Sensor domains of two-component regulatory systems.
Cheung et al. (2010)
9 more

3 mentions without citation

O2- and NO-sensing mechanism through the DevSR two-component system in Mycobacterium smegmatis.
Lee et al. (2008)
2 more