› 2vkv

X-ray diffraction
1.74Å resolution

TetR (BD) variant L17G with reverse phenotype

Released:
DOI: 10.2210/pdb2vkv/pdb
PDB entry 2vkv coloured by chain, front view.
PDB entry 2vkv coloured by chain, side view.
PDB entry 2vkv coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A protein functional leap: how a single mutation reverses the function of the transcription regulator TetR.
Resch M, Striegl H, Henssler EM, Sevvana M, Egerer-Sieber C, Schiltz E, Hillen W, Muller YA
Nucleic Acids Res 36 4390-401 (2008)
PMID: 18587152

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetracycline repressor protein class D Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 23.35 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACT4 (Residues: 1-208; Coverage: 95%)
Gene name: tetR
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand TDC 1 x TDC
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21212
Unit cell:
a: 70.65Å b: 54.46Å c: 56.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.175 0.225
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Functional Mechanism of the Efflux Pumps Transcription Regulators From Pseudomonas aeruginosa Based on 3D Structures.
Housseini B Issa et al. (2018)
4 more

1 mention without citation

A protein functional leap: how a single mutation reverses the function of the transcription regulator TetR.
Resch et al. (2008)