› 2vky

X-ray diffraction
2.05Å resolution

Headbinding Domain of Phage P22 Tailspike C-Terminally Fused to Isoleucine Zipper pIIGCN4 (Chimera I)

Released:
DOI: 10.2210/pdb2vky/pdb
PDB entry 2vky coloured by chain, front view.
PDB entry 2vky coloured by chain, side view.
PDB entry 2vky coloured by chain, top view.
Source organisms:
Primary publication:
Bacteriophage P22 tailspike: structure of the complete protein and function of the interdomain linker.
Seul A, Müller JJ, Andres D, Stettner E, Heinemann U, Seckler R
Acta Crystallogr D Biol Crystallogr 70 1336-45 (2014)
PMID: 24816102

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General control transcription factor GCN4; Tail spike protein Chain: B
Molecule details ›
Chain: B
Length: 154 amino acids
Theoretical weight: 17.22 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12528 (Residues: 2-125; Coverage: 19%)
  • Canonical: P03069 (Residues: 251-280; Coverage: 11%)
Gene names: 9, AAS101, AAS3, ARG9, GCN4, YEL009C
Sequence domains:
Structure domains: Phage P22 tailspike-like, N-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU H2B
Spacegroup: P63
Unit cell:
a: 57.81Å b: 57.81Å c: 107.12Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.147 0.145 0.187
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Targeting mechanisms of tailed bacteriophages.
Nobrega et al. (2018)
1 more

1 mention without citation

Dimerization of the SP1 Region of HIV-1 Gag Induces a Helical Conformation and Association into Helical Bundles: Implications for Particle Assembly.
Datta et al. (2016)