› 2vld

X-ray diffraction
2.6Å resolution

crystal structure of a repair endonuclease from Pyrococcus abyssi

Released:
DOI: 10.2210/pdb2vld/pdb
PDB entry 2vld coloured by chain, front view.
PDB entry 2vld coloured by chain, side view.
PDB entry 2vld coloured by chain, top view.
Source organism: Pyrococcus abyssi
Primary publication:
Structure and function of a novel endonuclease acting on branched DNA substrates.
Ren B, Kühn J, Meslet-Cladiere L, Briffotaux J, Norais C, Lavigne R, Flament D, Ladenstein R, Myllykallio H
EMBO J 28 2479-89 (2009)
PMID: 19609302

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194172 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease NucS Chains: A, B
Molecule details ›
Chains: A, B
Length: 251 amino acids
Theoretical weight: 29.15 KDa
Source organism: Pyrococcus abyssi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9V2E8 (Residues: 1-251; Coverage: 100%)
Gene names: PAB2263, PYRAB01260, nucS
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2221
Unit cell:
a: 78.966Å b: 100.688Å c: 157.686Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.243 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

The archaeal PCNA proteins.
Pan et al. (2011)
6 more

7 mentions without citation

PCNA-binding proteins in the archaea: novel functionality beyond the conserved core.
MacNeill SA. (2016)
6 more