› 2vnc

X-ray diffraction
3Å resolution

Crystal structure of Glycogen Debranching enzyme TreX from Sulfolobus solfataricus

Released:
DOI: 10.2210/pdb2vnc/pdb
PDB entry 2vnc coloured by chain, front view.
PDB entry 2vnc coloured by chain, side view.
PDB entry 2vnc coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Structural insight into the bifunctional mechanism of the glycogen-debranching enzyme TreX from the archaeon Sulfolobus solfataricus.
Woo EJ, Lee S, Cha H, Park JT, Yoon SM, Song HN, Park KH
J Biol Chem 283 28641-8 (2008)
PMID: 18703518

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161182 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 718 amino acids
Theoretical weight: 83.18 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: P95868 (Residues: 1-718; Coverage: 100%)
Gene names: SSOP1_2225, SULA_2828, SULB_2829, SULC_2826, SULG_14405, SULH_14405, SULI_14405, SULM_14335, SULN_14325, SULO_14345, SULZ_14425, glgX, orf c06021
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P3121
Unit cell:
a: 136.077Å b: 136.077Å c: 173.545Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.215 0.287
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure and function of α-glucan debranching enzymes.
Møller et al. (2016)
1 more

3 mentions without citation

Crystal structure of the Chlamydomonas starch debranching enzyme isoamylase ISA1 reveals insights into the mechanism of branch trimming and complex assembly.
Sim et al. (2014)
2 more