› 2vng

X-ray diffraction
1.4Å resolution

Family 51 carbohydrate binding module from a family 98 glycoside hydrolase produced by Clostridium perfringens in complex with blood group A-trisaccharide ligand.

Released:
DOI: 10.2210/pdb2vng/pdb
PDB entry 2vng coloured by chain, front view.
PDB entry 2vng coloured by chain, side view.
PDB entry 2vng coloured by chain, top view.
Source organism: Clostridium perfringens
Primary publication:
Divergent modes of glycan recognition by a new family of carbohydrate-binding modules.
Gregg KJ, Finn R, Abbott DW, Boraston AB
J Biol Chem 283 12604-13 (2008)
PMID: 18292090

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186907 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glycosyl hydrolase family 98 putative carbohydrate-binding module domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 19.88 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8XNK4 (Residues: 27-206; Coverage: 23%)
Gene name: CPE0329
Sequence domains: NPCBM/NEW2 domain
Structure domains: NPCBM/NEW2 domain

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL, FUC, A2G
Carbohydrate polymer
1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-002
Spacegroup: P21212
Unit cell:
a: 69.683Å b: 98.754Å c: 49.241Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Carbohydrate-binding domains: multiplicity of biological roles.
Guillén et al. (2010)
8 more

1 mention without citation

Divergent modes of glycan recognition by a new family of carbohydrate-binding modules.
Gregg et al. (2008)