› 2vnu

X-ray diffraction
2.3Å resolution

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170597 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Exosome complex exonuclease DIS3 Chain: D
Molecule details ›
Chain: D
Length: 760 amino acids
Theoretical weight: 87.04 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q08162 (Residues: 242-1001; Coverage: 76%)
Gene names: DIS3, O2197, RRP44, YOL021C
Sequence domains:
Structure domains:
5'-R(*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP)-3' Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.25 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand NA 1 x NA
Ligand 1PE 1 x 1PE
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 72.35Å b: 87.25Å c: 136.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

29 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
28 more

18 mentions without citation

The eukaryotic RNA exosome.
Januszyk et al. (2014)
17 more