› 2vof

X-ray diffraction
1.8Å resolution

Structure of mouse A1 bound to the Puma BH3-domain

Released:
DOI: 10.2210/pdb2vof/pdb
PDB entry 2vof coloured by chain, front view.
PDB entry 2vof coloured by chain, side view.
PDB entry 2vof coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural plasticity underpins promiscuous binding of the prosurvival protein A1.
Smits C, Czabotar PE, Hinds MG, Day CL
Structure 16 818-29 (2008)
PMID: 18462686

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170471 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bcl-2-related protein A1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 157 amino acids
Theoretical weight: 18.11 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q07440 (Residues: 1-152; Coverage: 88%)
Gene names: A1, Bcl2a1, Bcl2a1a, Bfl1
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Structure domains: Blc2-like
Bcl-2-binding component 3 Chains: B, D
Molecule details ›
Chains: B, D
Length: 26 amino acids
Theoretical weight: 3.19 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99ML1 (Residues: 130-155; Coverage: 14%)
Gene names: Bbc3, Puma

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 49.64Å b: 60.683Å c: 59.986Å
α: 90° β: 109.53° γ: 90°
R-values:
R R work R free
0.184 0.183 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Control of apoptosis by the BCL-2 protein family: implications for physiology and therapy.
Czabotar et al. (2014)
18 more

10 mentions without citation

Intrinsically disordered proteins in bcl-2 regulated apoptosis.
Rautureau et al. (2010)
9 more